12/22/2023 0 Comments Taurine and theanineUnder various stressful conditions, cells promote mTOR or other growth factors sensitive growth-stimulating pathways, causing cells to exhibit hyperactivity.įollowing damage to the genome and epigenome of cells, many senescence-related secretions are mainly transcribed through NF-κB, secreting interleukins, growth factors, proteases, cytokines, matrix-degrading enzymes, and metalloproteinases into the extracellular space. Proteotoxic stress, mitochondrial dysfunction, and genomic instability lead to cellular hyperactivity and are closely associated with age-related degenerative diseases such as atherosclerosis, type 2 diabetes, osteoporosis, and Alzheimer’s disease ( 1– 3). Our findings provide a new scientific basis for the relationship between tea drinking and brain protection. This study revealed for the first time that the synergistic effect of EGCG with L-theanine may be an effective way to promote nerve cell repair and regeneration and slow down the progression of AD. In addition, EGCG + L-theanine maintains the high-fidelity structure of cellular proteins. EGCG + L-theanine keeps differentiated nerve cells in a quiescent state, which is beneficial to the repair and regeneration of nerve cells. The synergistic mechanism study showed that under the premise that EGCG inhibits amyloid stress and inflammation and promotes metabolism, L-theanine could play a nourish nerve effect. EGCG + L-theanine inhibited inflammation and aggregate formation pathways, significantly increased the percentage of G0/G1 in the cell cycle, downregulated the expression of proteins such as p-mTOR, Cyclin D1, and Cyclin B1, upregulated the expression of GAP43, Klotho, p-AMPK, and other proteins, promoted mitochondrial activity and energy metabolism, and had repair and regeneration effects on differentiated nerve cells. The results showed that under Aβ 25–35 stress conditions, mitochondria and axons degenerated, and the expression of cyclins was up-regulated, showing the gene and protein characteristics of cellular hyperfunction. In this study, an AD model of Aβ 25–35-induced differentiated neural cell line PC12 cells was established to study the synergistic effect of EGCG and L-theanine in protecting neural cells. Epigallocatechin gallate (EGCG) and N-ethyl-L-glutamine ( L-theanine) are the representative functional components of green tea ( Camellia sinensis). Green tea has significant protective activity on nerve cells, but the mechanism of action is unclear. 3Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, China.2Key Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha, China.1National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, China.Xinya Xie 1,2,3 Juan Wan 1,2,3 Xin Zheng 1,2,3 Wenjing Pan 1,2,3 Jiayi Yuan 1,2,3 Baozhu Hu 1,2,3 Meiyan Feng 1,2,3 Zhonghua Liu 1,2,3* Shuxian Cai 1,2,3*
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